Biochemistry
reza Haji Hosseini
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
Introduction:
The aim of this study was to compare the total protein in serum of patient who was exposed to sulfur mustard (SM) during the Iran-Iraq war.
Materials and Methods:
This study included 35 patients exposed to mustard gas (case group) and 35 healthy controls were evaluated. Groups in serum ...
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Introduction:
The aim of this study was to compare the total protein in serum of patient who was exposed to sulfur mustard (SM) during the Iran-Iraq war.
Materials and Methods:
This study included 35 patients exposed to mustard gas (case group) and 35 healthy controls were evaluated. Groups in serum total protein was measured by the Bradford method.
Results:
The average amount of protein in the serum of exposure to mustard gas (patients) compared with a control group, showed no significant difference (05/0
Bioinformatics
Shamim Khandan Alamdari; Somayeh Farahmand; Reza Haji Hosseini; Gholamreza Bakhshi khaniki
Abstract
Human papillomavirus (HPV) is the most common sexually transmitted infection worldwide, and high-risk HPV types cause about five percent of all cancers worldwide. The chemical drugs used to treat this disease are expensive and have many side effects. Therefore, the use of herbal medicines is increasing. ...
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Human papillomavirus (HPV) is the most common sexually transmitted infection worldwide, and high-risk HPV types cause about five percent of all cancers worldwide. The chemical drugs used to treat this disease are expensive and have many side effects. Therefore, the use of herbal medicines is increasing. In this regard, the E6 protein, which is a key protein in the initiation of cervical cancer and plays a role in the degradation of P53, was selected as an essential drug target. In this research, two new potential inhibitors named beta-sitosterol (CID_222284) and loncocarpenin (CID_54699185) were identified as potent inhibitors of E6 HPV-16 from the PubChem library by high-throughput virtual screening. Molecular dynamics results show that these compounds bind to E6 protein with high stability. The preparation of ADMET and Swiss ADME profiles indicates that the identified compounds are probably potential candidates against E6 HPV-16 and can be used in chemotherapy by inhibiting the Pgp channel as an adjuvant drug.
Mostafa Robatsarpooshi; Reza Haji Hosseini; Majid Halimi Khalilabd; Gholam Reza Bakhshikhaniki
Abstract
Metal oxide nanoparticles, including Nano-silver, have antimicrobial effects against a wide range of microorganisms. The aim of this study was to investigate the effect of Nano-silver obtained from different parts of Saffron on Acinetobacter baumannii has always been identified as one of the most important ...
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Metal oxide nanoparticles, including Nano-silver, have antimicrobial effects against a wide range of microorganisms. The aim of this study was to investigate the effect of Nano-silver obtained from different parts of Saffron on Acinetobacter baumannii has always been identified as one of the most important nosocomial infections. In this study, saffron stigma, stamens, and petals were prepared, only stamens and petals caused the synthesis of Nano-silver. The diameter of Nanosilver was measured using TEM, then its effect on Acinetobacter baumannii was investigated by Disk and Well diffusion, MBC, and MIC methods. The results of the Disk diffusion method and the well diffusion method showed that with increasing the concentration of silver nanoparticles, the diameter of the bacterial growth aura increases. Also, the average MBC and MIC for saffron petal Nano-silver are 781 ppm and 390 ppm, respectively, and for saffron stamens Nano-silver, 3125 ppm, and 1562 ppm, respectively. It can be concluded that the petals and stamens of saffron reduce silver ions well and cause the synthesis of silver Nano-silver. The resulting Nano-silver also had a lethal effect on Acinetobacter baumannii. The abundance of saffron in Iran can be a good option for the production of Nano-silver.
Hamid Alibahar; Yousef Paridar; Maysam Mard-soltani; Reza Haji Hosseini
Abstract
Gastric cancer (GC) is one of the most common types of cancer and the second leading cause of cancer mortality. Delay in diagnosis and lack of screening are the main causes of high mortality from this disease. Finding an accurate and effective diagnostic biomarker seems to be essential for effective ...
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Gastric cancer (GC) is one of the most common types of cancer and the second leading cause of cancer mortality. Delay in diagnosis and lack of screening are the main causes of high mortality from this disease. Finding an accurate and effective diagnostic biomarker seems to be essential for effective treatment of GC. In this regard, using a gastroscope, we collected tissue samples from patients with GC and healthy individuals. The obtained samples were used to extract their RNA using Trizol solution kit. RNA samples were used for qRT-PCR using specific primers designed for BTG1 and GAPDH genes. QRT-PCR results were analyzed using 2-ΔCt method and various statistical tests using SPSS software. In total, 40 samples of GC and healthy controls were collected and their demographic information was recorded. RNA extraction produced the amount of RNA required for qRT-PCR. The qRT-PCR results showed that BTG1 expression was significantly decreased in GC samples. According to the obtained results, it can be concluded that the reduction of BTG1 expression can act as an accurate biomarker for GC. This gene can also be an indicator of GC pathogenicity. These results could indicate possible diagnostic and therapeutic applications of BTG1 for GC.
Leila Mirsadeghi; Reza Haji Hosseini; Ali Mohammad Banaei; Kaveh Kavousi
Abstract
The emergence of personalized medicine based on molecular techniques, such as next-generation sequencing, has increased our understanding of drivers of complex diseases, including cancers. In many cases due to the complexity of cancer, it is difficult for human physicians and biologists to make decisions ...
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The emergence of personalized medicine based on molecular techniques, such as next-generation sequencing, has increased our understanding of drivers of complex diseases, including cancers. In many cases due to the complexity of cancer, it is difficult for human physicians and biologists to make decisions on the basis solely of clinical practice or laboratory evidence. Thus, the personalized medicine approach comes into play and provides large volumes and valuable data for experts. Further, data analysis with bioinformatic tools has opened a new horizon in the process of prognosis and screening of in risk individuals. It has caused significant recent advances in diagnostic technology and improved targeted treatments. In the present study, archived formalin-fixed paraffin-embedded tissue from an Iranian female patient with invasive breast carcinoma was investigated. In this way, after DNA extraction and purification, the whole exome was sequenced and the mutation data were analyzed. Obtained information could help to the enrichment of the Iranian genome databases. In the light of this research and by studying other Iranian samples, we can provide an optimized roadmap for precision oncologists to increase the life expectancy of breast cancer patients.
Shahrzad Fouladi; Reza Haji Hosseini; Masoud Torkzadeh-Mahani; Hooshang Hamidian
Abstract
Urate oxidase or uricase (UOX) (EC 1.7.3.3) is an globular tetramer oxidoreductase enzyme which lacks cofactor. Much research on this enzyme has been done so far due to its biomedical applications as a therapeutic and diagnostic agent. Urate oxidase catalyzes uric acid degradation and produces allantoin. ...
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Urate oxidase or uricase (UOX) (EC 1.7.3.3) is an globular tetramer oxidoreductase enzyme which lacks cofactor. Much research on this enzyme has been done so far due to its biomedical applications as a therapeutic and diagnostic agent. Urate oxidase catalyzes uric acid degradation and produces allantoin. An imbalance in the excretion of uric acid causes the hyperuricemia. Part of a clinically important diagnostic kit used to measure the concentration of uric acid in the blood is the enzyme uricase. In this research, efforts are made to investigate the effect of triethylammonium maleate (TEAM) ionic liquid on the function of urate oxidase (UOX). Ionic liquids are salts with important properties like high thermal stability, high solvating capacity and high polarity. These salts have been considered in biochemical and biomedical fields. They can also be utilized as a stabilizer for long-term protein storage and they can extend the shelf life of some proteins such as therapeutic or industrial proteins. In this study, we treated the enzyme in different concentrations of triethylammonium maleate ionic liquid. The volume percentages of TEAM in the solvent phase are 1%, 2%, 5%, 10% and 15%. The results indicate that TEAM has a concentration-dependent effect on the activity of UOX enzyme. The use of 5% TEAM ionic liquid increased the enzymatic activity in comparison to untreated enzyme. We concluded that this ionic liquid was able to alter the structure of the uricase in a way that increased the activity and improve its catalytic efficiency of uricase.
Shahrzad Fouladi; Reza Haji Hosseini; Masoud Torkzadeh; Hooshang Hamidian
Abstract
Ionic liquids (ILs) are salts that can affect the structure, stability, and function of proteins. Researchers have recently been interested in finding ionic liquids that increase the stability, activity and solubility of enzymes. In this study, efforts are made to investigate the effect of triethylammonium ...
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Ionic liquids (ILs) are salts that can affect the structure, stability, and function of proteins. Researchers have recently been interested in finding ionic liquids that increase the stability, activity and solubility of enzymes. In this study, efforts are made to investigate the effect of triethylammonium propionate (TEAP) on the function of urate oxidase (UOX). We treated the enzyme in different concentrations of TEAP. The volume percentages of TEAP in the solvent phase are 0.5%, 1%, 2%, 5%, 10% and 15%. The results indicate that TEAP has a concentration-dependent effect on the activity of UOX enzyme. The use of 1% TEAP ionic liquid increased the enzymatic activity in comparison to untreated enzyme. We concluded that this ionic liquide was able to alter the structure of the uricase in a way that increased the activity and improve its catalytic efficiency of the enzyme. Also the thermodynamic prameters such as ΔG#, ΔH#, and ΔS# values indicate that the use of 1% of triethylammonium propionate increases the thermostability of uricase and reduces the conformational changes of this enzyme during thermal inactivation process.
Fariba Yaghmori; Reza Hajihosseini; Seyed Mehrdad Kassaee; Bahram Seifizarei
Abstract
Abstract Non-steroidal anti-inflammatory drugs (NSAIDs) have beneficial effects in numerous experimental models of sepsis. Therefore, in the present study, we investigated the protective effects of three NSAIDs against sepsis induced liver damages. A total number of 60 male Wistar rats were randomly ...
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Abstract Non-steroidal anti-inflammatory drugs (NSAIDs) have beneficial effects in numerous experimental models of sepsis. Therefore, in the present study, we investigated the protective effects of three NSAIDs against sepsis induced liver damages. A total number of 60 male Wistar rats were randomly divided into six experimental groups. Rats were treated with indomethacin, celecoxib and aspirin orally with dose of 2 mg/kg b.w for 48h after CLP (Cecal ligation and puncture) injury. Then, blood samples were collected to determine serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and Bilirubin. The extracted livers were used for biochemical assays [Malondialdehyde (MDA), glutathione (GSH) and myeloperoxidase (MPO) activities] and real time PCR studies. The result revealed that treatments significantly improved antioxidant and liver enzymes by reducing MDA, MPO, AST and ALT level and increasing level of GSH. Moreover, cyclooxygenase-2(COX-2) expression in the liver tissue was decreased in the treatment group compared to the CLP group. Thus, our result suggests CLP induced oxidative hepatic damage and NSAIDs have the potential for the treatment of liver damage consecutive to chemical intoxication.
Faezeh fatemi; Reza Hajihosseini; Abbas Golbodagh; abolfazl dadkhah
Abstract
Abstract Sepsis is the most common reason of mortality among patients who are in the intensive care unit. Regarding to the side effects of the anti-inflammatory drug consumption, the replace of natural products are suggested in sepsis treatment. the effects of the combination of the deuterium depleted ...
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Abstract Sepsis is the most common reason of mortality among patients who are in the intensive care unit. Regarding to the side effects of the anti-inflammatory drug consumption, the replace of natural products are suggested in sepsis treatment. the effects of the combination of the deuterium depleted water (DDW) and Rosa (R.) damascena Mill. on the stress oxidative parameters and the gene expression of cyclooxygenase-2 (COX-2) in the lung and plasma tissues were investigated. 50 Rats were randomly divided into 5 groups: negative control (LAP), control group (CLP), two treatment groups with the combination of DDW and R. damascena Mill. essential oil (DDW15+EO and DDW30+EO) and positive control group with indomethacin (IND). Then, the levels of oxidative stress parameters and the expression of COX-2 were estimated in plasma and lung tissue. The sepsis resulted in the decrease of ferric reducing antioxidant power (FRAP) and glutathione (GSH) levels along with the increase of lipid peroxidation (LP) andCOX-2 levels. However, the rats treated with the combination of deuterium depleted water and R. damascena Mill. essential oils as same as indomethacin were influenced on the regulation of those parameters through the evaluation of FRAP and GSH levels and the reduction of the LP level and COX-2 gene expression. The pathological studies confirmed the biochemical consequences as well. The results indicated that the oxidative damages were caused by sepsis, but the administration of the natural products such as deuterium depleted water and R. damascena Mill. essential oils could improve the injures due to the effectiveness of oxidative stress and antioxidants parameters.
Reza Hajihosseini; Akram Bayati; Azadeh Rasooli; Atoosa Vaziri
Volume 6, Issue 3 , March 2018, , Pages 49-59
Abstract
Background and Objective: In the present study, we investigated the protective effects of Zataria multiflora essential oil against iron nano particle induced liver damage in rats. The animals were divided into three groups: negative control group; animals were treated with normal salin in three days. ...
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Background and Objective: In the present study, we investigated the protective effects of Zataria multiflora essential oil against iron nano particle induced liver damage in rats. The animals were divided into three groups: negative control group; animals were treated with normal salin in three days. poitive control group: 200 mg/kg b.w iron nano particle was administered intrapertoneally to male in three days. Treatment group with Zataria multiflora oil; animals were injected intraperitoneally with Zataria multiflora oils (100 and 200 mg/kg b.w) in three days. After three days, blood samples were drawn from heart and the liver tissues were removed for biochemical and histological studies. The hepatoprotective activity was assessed using various biochemical parameters such as alanine aminotransferase (ALT), aspartate amino transferas (AST), alkalin phosphatase (ALP), Ferric Reducing Ability of Plasma (FRAP), lipid peroxidation (LP) and glutathione (GSH). The results of this study showed that iron nano particles caused increasing the serum AST and LP levels and decreasing FRAP and GSH levels, but it has no effect on ALT and ALP. Consumption of Zataria multiflora oil reversed AST, LP and GSH levels to the normal levels without any effect on FRAP. Finally, treatment of rats with iron nanoparticle caused induction of oxidative hepatic damage and consumption of zataria essential oil can be effective for the recovery and prevent of liver damage.
Shiva Nemati; Ebrahim Shahbazi; Mehdi Hesaraki; Sahar Kiani; Reza Haji Hossein
Volume 5, Issue 2 , December 2016, , Pages 35-51
Abstract
Spinal cord injury (SCI) is a devastating condition producing great personal and societal costs and for which there is no effective treatment. Stem cell transplantation is a promising therapeutic strategy, though much preclinical and clinical research work remains. Here, we briefly describe SCI epidemiology, ...
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Spinal cord injury (SCI) is a devastating condition producing great personal and societal costs and for which there is no effective treatment. Stem cell transplantation is a promising therapeutic strategy, though much preclinical and clinical research work remains. Here, we briefly describe SCI epidemiology, pathophysiology, and transplantation trial in human developments, including termination of the first human embryonic stem cell experimental and clinical stem cell strategies. Research in stem cell biology and cell reprogramming is rapidly advancing, with the hope of moving stem cell therapy closer to helping people with SCI. We examine issue important for clinical translation and provide a commentary on recent SCI.
Shahla Roozbahani; Fatemeh Alipourfard
Volume 4, Issue 2 , December 2015, , Pages 1-6
Abstract
Cytotoxic effect of extract leaves of T. kotschyanus Boiss. & Hohen on Human Lang Cell. T. kotschyanus BOISS. & Hohen was used in traditional medicine such as anti-inflammation, antispasmodic and gastrointestinal aim of this study was to survey the cytotoxic effects the of ethanol of leave of ...
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Cytotoxic effect of extract leaves of T. kotschyanus Boiss. & Hohen on Human Lang Cell. T. kotschyanus BOISS. & Hohen was used in traditional medicine such as anti-inflammation, antispasmodic and gastrointestinal aim of this study was to survey the cytotoxic effects the of ethanol of leave of thymus kotschyanus on A-549 cell line. T. kotschyanus was collected on spring 2014 from Selseleh regions of Lorestan province leaves plant for 3 days at room temperature. Dried herd powder was and extract ethanol. After preparing extract 25, 50, 100, 20, 500 μg/ml concentration for extract leveas T. kotschyanus on the cells were evaluated for 7 2 hours. Cytotoxic effects of T. kotschyanus extract leveas T. kotschyanus against cancer cell was measured by using SPSS. Results shows that, the extracts leaves ethanol T. kotschyanus has cytotoxic effects on A-549 cell lien. The finding suggest that extracts ethanol leaves T. kotschyanus to existence phenolic compounds, especially flavonoids has an inhibitory effect on the A-549 cell line.
P. Boloori; R. Haji Hosseini; R. Shekh-Nejad; M. Hamedani; M. Sajadi; Z. Farsad
Volume 3, Issue 3 , May 2015, , Pages 1-12
Abstract
Abstract The altered or mutated forms of genes known as proto-oncogenes are responsible for promoting cell growth and uncontrolled cell proliferation. An accumulation of many mutations in different and specific genes,over time is required to cause cancer. The pattern of gene expression, also called ...
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Abstract The altered or mutated forms of genes known as proto-oncogenes are responsible for promoting cell growth and uncontrolled cell proliferation. An accumulation of many mutations in different and specific genes,over time is required to cause cancer. The pattern of gene expression, also called molecular signature is unique to a particular class of tumor or tumor cell. This paper describes the latest technique for monitoring the expression of a panel of cancer-specific genes. The PCR technique combines the quantitative performance of SYBR® Green-based real-time PCR is widely used for gene profiling. This technique is cost-effective, easy-to-use, and focuses only on the genes that you desire. In this study the expression of our target genes were quantitatively determined in five human cancer cell lines. We selected gene β-actin as our reference gene. Cells were lysed and the mRNAs were extracted using the RNA Purification Kit and cleaned up with Qiagen RNeasy spin columns. The first-strand cDNA was synthesized according to the High Capacity cDNA Reverse Transcription Kit protocol. RT-PCR were performed with Gene Expression Assays in an AB step one plus Sequence Detection System. Briefly the expression of p53 was high in both breast cancer cell lines, MCF7, T47-D and lung cancer cells, A549. Src expression was higher in prostate cell line, PC3 and lung cancer cells, A549. Meanwhile SKOV3 (ovarian cancer cell line) showed high expression of her-2 gene. The results clearly show that the expression pattern of this panel of genes was unique to almost every cell line examined.
M. A. Esfandiari; M. Shohrati; R. Hajihosseini; M. Naderi4; A. Gholbodagh; M. Ghodarzi
Volume 2, Issue 2 , September 2014, , Pages 53-60
Abstract
The aim of this study was to compare the total protein levels in serum of veteran exposure to sulfur mustard (SM) which was used in the Iran-Iraq war. In this study, 35 patients exposed to mustard gas (case group) and 35 healthy subjects (control group) were evaluated. The total protein concentration ...
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The aim of this study was to compare the total protein levels in serum of veteran exposure to sulfur mustard (SM) which was used in the Iran-Iraq war. In this study, 35 patients exposed to mustard gas (case group) and 35 healthy subjects (control group) were evaluated. The total protein concentration was measured by Bradford method. The average amount of protein in serum exposure to sulfur mustard (patients) in compared with a control group, showed no significant differences (p>0.05). The observed inflammatory reaction in the lungs and eyes exposed veterans with sulfur mustard gas and the total protein in the serum of healthy individual veterans did not show significant differences in inflammatory factor. Therefore, it should be the amounts of inflammatory factors in local (lungs and eyes) were high and the systemic inflammatory factors may be increased.
